Column chromatography silica gel
Column chromatography silica gel is one of the highly acclaimed techniques for separation as well as analysis of various complex organic mixtures.We are one of the major manufacturers of column chromatography silica gel. In order to meet different needs of customers, we offer column chromatography silica gel in various particle sizes for pilot plant or other plant applications.
With the exception of TLC, columns are used for the separation step in all chromatographic techniques. Many laboratories now use column chromatography for both prepping samples and monitoring reactions during organic synthesis. The following reasons are the main causes of column chromatography’s significance:
Simple packing procedure
Low operating pressure
Low expense for instrumentation
There are two types of column chromatography, according to the way how the solvent flows down the column. Gravity column chromatography, also known as percolation, is the process of allowing the solvent to flow down the column by gravity. The “state of the art” technique is called flash chromatography if the solvent is pushed down the column by positive air pressure. Currently, research labs for organic chemistry employ this method.
For column chromatography, organic chemists frequently utilize silica gel (SiO2) and alumina (Al2O3) as adsorbents. These adsorbents come in various mesh sizes, which are identified by numbers on the bottle label; for example “silica gel 60” and “silica gel 230-400.” Specifically, this number relates to the number of holes in the mesh or sieve that the raw silica particle combination passes through during the production process.
The solvent flows differently through the column depending on the adsorbent particle size. For flash chromatography, smaller particles with higher mesh values are applied; for gravity chromatography, larger particles with lower mesh values are utilized. For gravity columns, 70–230 silica gels are applied, whereas flash columns use 230–400 mesh.
Features and Benefits
Batch to Batch reproducible results
Excellent flow rates
Typical Data of Silica gel Column Grade Adsorbents
Iron Content – < 0.02%
Chloride Content – < 0.10%
Loss on Drying – <3%
pH (10% suspension) – 7 ± 0.5
Surface Area – 400–600 m2/gm